Formulation and Evaluation of Midazolam loaded Hydrogel as a Carrier for Nose to Brain Drug Delivery

3/11/2020 14:40 - 15:15

Midazolam is a hypnotic, anxiolytic, anticonvulsant and sedative drug used in children to control seizure. Intranasal midazolam also suppresses epileptic activity and improves the background of electroencephalograms in children with epilepsy. The present study was designed to prepare midazolam loaded intranasal thermo responsive hydrogel and its characterization. Drug loaded pluronic and carbopol based hydrogel was prepared by cold method. Depending upon the concentration of polymers six formulations were prepared. The prepared hydrogels were characterized for gelation temperature, viscosity, entrapment efficiency, and drug release. To assess the inter linked structure of the whole hydrogel formulations, X-ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FTIR) and scanning electron microscopy (SEM) were performed. Drug permeation study was carried out in buffer solution (pH 5.8) by Franz diffusion cell. Intranasal hydrogel shows 25.7℃ to 28.2℃ gelation temperature which is in acceptable range of 25℃ to 35℃. Viscosity studies show that higher the concentration of pluronic, higher was the viscosity (100.9 to 126.1) and the lower the gelling temperature (25.7℃ to 28.2℃). pH of formulations was 5.8 to 6.0 which is in physiological pH range. Entrapment efficiency of all formulations were observed between 68.7% to 79%. In vitro release study shown that no formulation follows zero order kinetics except one formulation. Regression coefficient (n) of all formulations falls from 0.520 to 0.728 indicates that release of drug is non-Fickian. It is concluded that midazolam loaded hydrogel was successfully prepared by cold method with good entrapment efficiency and that carbopol/pluronic based hydrogel is a good vehicle for nose to brain drug delivery.

Muhammad Akhtar, Associate Professor at Faculty of Pharmacy and Alternative Medicine, The Islamia University of Bahawalpur